Chapter title |
Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants
|
---|---|
Chapter number | 22 |
Book title |
Plant Germline Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7286-9_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7285-2, 978-1-4939-7286-9
|
Authors |
Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, Lynette Brownfield, Peters, Benjamin, Aidley, Jack, Cadzow, Murray, Twell, David, Brownfield, Lynette |
Abstract |
The male germline of flowering plants develops within the vegetative cell of the male gametophyte and displays a distinct transcriptional profile. Key to understanding the development of this unique cell lineage is determining how gene expression is regulated within germline cells. This knowledge impacts upon our understanding of cell specification, differentiation, and plant fertility. Here, we describe methods to identify cis-regulatory modules (CRMs) that act as key regulatory regions in the promoters of germline-expressed genes. We detail the complimentary techniques of phylogenetic footprinting and the use of fluorescent reporters in pollen for the identification and verification of CRMs. |
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