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Plant Germline Development

Overview of attention for book
Cover of 'Plant Germline Development'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 An Introduction to Male Germline Development
  3. Altmetric Badge
    Chapter 2 Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants
  4. Altmetric Badge
    Chapter 3 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice
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    Chapter 4 Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana
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    Chapter 5 Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers
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    Chapter 6 Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy
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    Chapter 7 In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes
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    Chapter 8 Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins
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    Chapter 9 Live-Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes
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    Chapter 10 Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy
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    Chapter 11 Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures
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    Chapter 12 Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues
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    Chapter 13 Whole Mount RNA-FISH on Ovules and Developing Seeds
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    Chapter 14 Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy
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    Chapter 15 Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes
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    Chapter 16 Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS)
  18. Altmetric Badge
    Chapter 17 Isolation of Rice Sperm Cells for Transcriptional Profiling
  19. Altmetric Badge
    Chapter 18 Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation
  20. Altmetric Badge
    Chapter 19 Isolation and Detection of Small RNAs from Pollen
  21. Altmetric Badge
    Chapter 20 Analysis of Proteins Enriched in Rice Gamete
  22. Altmetric Badge
    Chapter 21 Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract
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    Chapter 22 Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants
  24. Altmetric Badge
    Chapter 23 RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis
  25. Altmetric Badge
    Chapter 24 RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data
  26. Altmetric Badge
    Chapter 25 Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality
  27. Altmetric Badge
    Chapter 26 Agrobacterium-Mediated Sorghum Transformation
  28. Altmetric Badge
    Chapter 27 Use of the Cas9 Orthologs from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana
  29. Altmetric Badge
    Chapter 28 Transgenic Reproductive Cell Ablation
  30. Altmetric Badge
    Chapter 29 In Vivo Reporters for Protein Half-Life
  31. Altmetric Badge
    Chapter 30 Conditional Modulation of Biological Processes by Low-Temperature Degrons
Attention for Chapter 25: Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality
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Chapter title
Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality
Chapter number 25
Book title
Plant Germline Development
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7286-9_25
Pubmed ID
Book ISBNs
978-1-4939-7285-2, 978-1-4939-7286-9
Authors

Iris Heidmann, Marco Di Berardino, Heidmann, Iris, Berardino, Marco

Abstract

Analyzing pollen quality in an efficient and reliable manner is of great importance to the industries involved in seed and fruit production, plant breeding, and plant research. Pollen quality parameters, viability and germination capacity, are analyzed by various staining methods or by in vitro germination assays, respectively. These methods are time-consuming, species-dependent, and require a lab environment. Furthermore, the obtained viability data are often poorly related to in vivo pollen germination and seed set. Here, we describe a quick, label-free method to analyze pollen using microfluidic chips inserted into an impedance flow cytometer (IFC). Using this approach, pollen quality parameters are determined by a single measurement in a species-independent manner. The advantage of this protocol is that pollen viability and germination can be analyzed quickly by a reliable and standardized method.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 24%
Researcher 5 20%
Student > Bachelor 2 8%
Other 1 4%
Student > Ph. D. Student 1 4%
Other 3 12%
Unknown 7 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 48%
Unspecified 1 4%
Biochemistry, Genetics and Molecular Biology 1 4%
Physics and Astronomy 1 4%
Medicine and Dentistry 1 4%
Other 1 4%
Unknown 8 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 May 2018.
All research outputs
#14,081,725
of 23,003,906 outputs
Outputs from Methods in molecular biology
#3,963
of 13,158 outputs
Outputs of similar age
#223,433
of 421,219 outputs
Outputs of similar age from Methods in molecular biology
#346
of 1,074 outputs
Altmetric has tracked 23,003,906 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,158 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,219 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.