Chapter title |
Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality
|
---|---|
Chapter number | 25 |
Book title |
Plant Germline Development
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7286-9_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7285-2, 978-1-4939-7286-9
|
Authors |
Iris Heidmann, Marco Di Berardino, Heidmann, Iris, Berardino, Marco |
Abstract |
Analyzing pollen quality in an efficient and reliable manner is of great importance to the industries involved in seed and fruit production, plant breeding, and plant research. Pollen quality parameters, viability and germination capacity, are analyzed by various staining methods or by in vitro germination assays, respectively. These methods are time-consuming, species-dependent, and require a lab environment. Furthermore, the obtained viability data are often poorly related to in vivo pollen germination and seed set. Here, we describe a quick, label-free method to analyze pollen using microfluidic chips inserted into an impedance flow cytometer (IFC). Using this approach, pollen quality parameters are determined by a single measurement in a species-independent manner. The advantage of this protocol is that pollen viability and germination can be analyzed quickly by a reliable and standardized method. |
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Demographic breakdown
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Members of the public | 3 | 100% |
Mendeley readers
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Other | 1 | 4% |
Unknown | 8 | 32% |