Chapter title |
Isothermal Titration Calorimetry to Determine Apparent Dissociation Constants (K d) and Stoichiometry of Interaction (n) of C-di-GMP Binding Proteins
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Chapter number | 30 |
Book title |
c-di-GMP Signaling
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7240-1_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7239-5, 978-1-4939-7240-1
|
Authors |
Bruno Y. Matsuyama, Petya V. Krasteva, Marcos V. A. S. Navarro, Matsuyama, Bruno Y., Krasteva, Petya V., Navarro, Marcos V. A. S. |
Abstract |
Isothermal titration calorimetry (ITC) is a commonly used biophysical technique that enables the quantitative characterization of intermolecular interactions in solution. Based on enthalpy changes (ΔH) upon titration of the binding partner (e.g., a small-molecule ligand such as c-di-GMP) to the molecule of interest (e.g., a receptor protein), the resulting binding isotherms provide information on the equilibrium association/dissociation constants (K a, K d) and stoichiometry of binding (n), as well as on changes in the Gibbs free energy (ΔG) and entropy (ΔS) along the interaction. Here we present ITC experiments used for the characterization of c-di-GMP binding proteins and discuss advantages and potential caveats in the interpretation of results. |
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