Chapter title |
Live Flow Cytometry Analysis of c-di-GMP Levels in Single Cell Populations
|
---|---|
Chapter number | 10 |
Book title |
c-di-GMP Signaling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7240-1_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7239-5, 978-1-4939-7240-1
|
Authors |
Jongchan Yeo, Xin C. Wang, Ming C. Hammond |
Abstract |
Second-generation RNA-based fluorescent biosensors have been developed that enable flow cytometry experiments to monitor the population dynamics of c-di-GMP signaling in live bacteria. These experiments are high-throughput, provide information at the single-cell level, and can be performed on cells grown in complex media and/or under anaerobic conditions. Here, we describe flow cytometry methods for three applications: (1) high-throughput screening for diguanylate cyclase activity, (2) analyzing c-di-GMP levels under anaerobic conditions, and (3) monitoring cell population dynamics of c-di-GMP levels upon environmental changes. These methods showcase RNA-based fluorescent biosensors as versatile tools for studying c-di-GMP signaling in bacteria. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 30% |
Student > Doctoral Student | 2 | 20% |
Other | 1 | 10% |
Student > Postgraduate | 1 | 10% |
Unknown | 3 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 3 | 30% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Agricultural and Biological Sciences | 1 | 10% |
Chemistry | 1 | 10% |
Unknown | 3 | 30% |