| Chapter title |
Chromatin Immunoprecipitation Analysis of Bortezomib-Mediated Inhibition of NFκB Recruitment to IL-1β and TNFα Gene Promoters in Human Macrophages.
|
|---|---|
| Chapter number | 29 |
| Book title |
Cytokine Bioassays
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4939-0928-5_29 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0927-8, 978-1-4939-0928-5
|
| Authors |
Shannon Sanacora, Tzu-Pei Chang, Ivana Vancurova, Sanacora S, Chang TP, Vancurova I |
| Abstract |
Interleukin-1β (IL-1) and tumor necrosis factor-α (TNF) are important pro-inflammatory cytokines involved in the mediation of the immune response, inflammation, tissue repair, and tumor progression. Regulation of IL-1 and TNF expression is mediated at the level of transcription by the transcription factor NFκB. Inhibition of NFκB activity by the proteasome inhibitor bortezomib (BZ) has been used as a frontline therapy in multiple myeloma and other hematological malignancies. In this chapter, we describe a protocol that uses chromatin immunoprecipitation (ChIP) to analyze the NFκB recruitment to endogenous IL-1 and TNF promoters in BZ-treated human macrophages. Corresponding to the BZ-suppressed mRNA levels of IL-1 and TNF, we show that BZ inhibits p65 NFκB recruitment to IL-1 and TNF promoters. This study specifically uses U937 macrophages, but the protocol could be easily modified to analyze the regulation of NFκB recruitment in other cell types. |
X Demographics
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|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 43% |
| Unspecified | 1 | 14% |
| Other | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 14% |
| Agricultural and Biological Sciences | 1 | 14% |
| Immunology and Microbiology | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Unknown | 3 | 43% |