↓ Skip to main content

ErbB Receptor Signaling

Overview of attention for book
Cover of 'ErbB Receptor Signaling'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 ErbB Receptors and Cancer
  3. Altmetric Badge
    Chapter 2 New Insights from Drosophila into the Regulation of EGFR Signaling
  4. Altmetric Badge
    Chapter 3 C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking
  5. Altmetric Badge
    Chapter 4 Targeting HER2 in Advanced Breast Cancer
  6. Altmetric Badge
    Chapter 5 Methods to Investigate EGFR Ubiquitination
  7. Altmetric Badge
    Chapter 6 Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent
  8. Altmetric Badge
    Chapter 7 Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases
  9. Altmetric Badge
    Chapter 8 Activation of Endosome-Associated Inert EGF Receptor Following Internalization
  10. Altmetric Badge
    Chapter 9 Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation
  11. Altmetric Badge
    Chapter 10 Study of EGFR Signaling/Endocytosis by Site-Directed Mutagenesis
  12. Altmetric Badge
    Chapter 11 Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR
  13. Altmetric Badge
    Chapter 12 Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay
  14. Altmetric Badge
    Chapter 13 Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway Activation
  15. Altmetric Badge
    Chapter 14 Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells
  16. Altmetric Badge
    Chapter 15 Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures
  17. Altmetric Badge
    Chapter 16 Studying Nonproliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo
  18. Altmetric Badge
    Chapter 17 Analysis of Epithelial–Mesenchymal Transition Induced by Overexpression of Twist
  19. Altmetric Badge
    Chapter 18 Assessment of Specificity of an Adenovirus Targeted to HER3/4
  20. Altmetric Badge
    Chapter 19 Isolation of Human Mesenchymal Stem Cells for Studying ErbB Receptor Signaling
Attention for Chapter 5: Methods to Investigate EGFR Ubiquitination
Altmetric Badge

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
16 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Methods to Investigate EGFR Ubiquitination
Chapter number 5
Book title
ErbB Receptor Signaling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7219-7_5
Pubmed ID
Book ISBNs
978-1-4939-7218-0, 978-1-4939-7219-7
Authors

Alexia Conte, Sara Sigismund

Abstract

Ubiquitination of the epidermal growth factor receptor (EGFR) is an important intracellular signal that occurs upon EGF stimulation and controls EGFR trafficking at multiple steps, finally destining the receptor to lysosomal degradation. In this chapter, we give an overview of the biochemical methods to investigate EGFR ubiquitination.Firstly, we describe the in vitro ubiquitination assay, a method where, in the presence of the minimal ubiquitination machinery, the biological milieu for EGFR ubiquitination is reproduced in a test tube. In the second protocol, we explain how to immunoprecipitate the EGFR from total lysate and reveal its ubiquitinated form by western blot analysis. Then, with an ELISA-derived assay, we illustrate a robust and reliable method to assess EGFR ubiquitination from low amount of sample; lastly, we illustrate an immunofluorescence protocol to visualize ubiquitinated species (including the EGFR itself) within the EGFR-positive endocytic compartments upon EGF stimulation.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 25%
Student > Ph. D. Student 3 19%
Other 2 13%
Student > Master 2 13%
Student > Bachelor 1 6%
Other 1 6%
Unknown 3 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 44%
Chemistry 2 13%
Agricultural and Biological Sciences 1 6%
Medicine and Dentistry 1 6%
Immunology and Microbiology 1 6%
Other 0 0%
Unknown 4 25%