↓ Skip to main content

ErbB Receptor Signaling

Overview of attention for book
Cover of 'ErbB Receptor Signaling'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 ErbB Receptors and Cancer
  3. Altmetric Badge
    Chapter 2 New Insights from Drosophila into the Regulation of EGFR Signaling
  4. Altmetric Badge
    Chapter 3 C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking
  5. Altmetric Badge
    Chapter 4 Targeting HER2 in Advanced Breast Cancer
  6. Altmetric Badge
    Chapter 5 Methods to Investigate EGFR Ubiquitination
  7. Altmetric Badge
    Chapter 6 Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent
  8. Altmetric Badge
    Chapter 7 Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases
  9. Altmetric Badge
    Chapter 8 Activation of Endosome-Associated Inert EGF Receptor Following Internalization
  10. Altmetric Badge
    Chapter 9 Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation
  11. Altmetric Badge
    Chapter 10 Study of EGFR Signaling/Endocytosis by Site-Directed Mutagenesis
  12. Altmetric Badge
    Chapter 11 Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR
  13. Altmetric Badge
    Chapter 12 Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay
  14. Altmetric Badge
    Chapter 13 Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway Activation
  15. Altmetric Badge
    Chapter 14 Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells
  16. Altmetric Badge
    Chapter 15 Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures
  17. Altmetric Badge
    Chapter 16 Studying Nonproliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo
  18. Altmetric Badge
    Chapter 17 Analysis of Epithelial–Mesenchymal Transition Induced by Overexpression of Twist
  19. Altmetric Badge
    Chapter 18 Assessment of Specificity of an Adenovirus Targeted to HER3/4
  20. Altmetric Badge
    Chapter 19 Isolation of Human Mesenchymal Stem Cells for Studying ErbB Receptor Signaling
Attention for Chapter 8: Activation of Endosome-Associated Inert EGF Receptor Following Internalization
Altmetric Badge

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
5 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Activation of Endosome-Associated Inert EGF Receptor Following Internalization
Chapter number 8
Book title
ErbB Receptor Signaling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7219-7_8
Pubmed ID
Book ISBNs
978-1-4939-7218-0, 978-1-4939-7219-7
Authors

Yi Wang, Sukhmani Billing, Zhixiang Wang

Abstract

Our current understanding of endosomal signaling is incomplete given our inability to generate endosomal signals in isolation from plasma membrane signals. We present a novel method to specifically activate epidermal growth factor (EGF) receptor (EGFR) following its internalization into endosomes. This method will allow us to address questions such as whether endosomal signaling is sufficient to activate signal transduction pathways and produce biological responses. In this method, the cells are treated with EGF in the presence of AG1478, a specific EGFR tyrosine kinase inhibitor, and monensin, which blocks recycling of EGFR. The treatment results in the internalization of nonactivated EGF-EGFR complex into endosomes. Next, the removal of AG1478 and monensin causes endosome-associated EGFR activation. Phosphorylated surface EGFR levels are undetectable during the treatment. This is a unique and novel approach to study endosomal signaling and its physiological relevance. The protocol is transferable to study endosomal signaling of other receptor tyrosine kinases.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 20%
Researcher 1 20%
Unknown 3 60%
Readers by discipline Count As %
Computer Science 1 20%
Immunology and Microbiology 1 20%
Unknown 3 60%