Chapter title |
Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer
|
---|---|
Chapter number | 16 |
Book title |
Acute Myeloid Leukemia
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7142-8_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7140-4, 978-1-4939-7142-8
|
Authors |
Hong, Chang-Sook, Funk, Sonja, Whiteside, Theresa L., Chang-Sook Hong, Sonja Funk, Theresa L. Whiteside |
Abstract |
A method for exosome isolation from human plasma was developed for rapid, high-throughput processing of plasma specimens obtained from patients with cancer. This method removes the bulk of plasma proteins associated with exosomes and can be used for comparative examinations of exosomes and their content in serial specimens of patients' plasma, allowing for monitoring changes in exosome numbers, profiles, and functions in the course of cancer progression or during therapy. The plasma-derived exosomes can be recovered in quantities sufficient for the characterization of their morphology by transmission electron microscopy (TEM), size and concentration by qNano, protein/lipid ratios, nucleic acid extraction, molecular profiling by Western blots or immune arrays, and functional assays. |
X Demographics
Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 81 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 16 | 20% |
Student > Ph. D. Student | 14 | 17% |
Student > Master | 13 | 16% |
Student > Bachelor | 7 | 9% |
Unspecified | 3 | 4% |
Other | 11 | 14% |
Unknown | 17 | 21% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 22 | 27% |
Agricultural and Biological Sciences | 12 | 15% |
Medicine and Dentistry | 11 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
Unspecified | 3 | 4% |
Other | 11 | 14% |
Unknown | 19 | 23% |