Chapter title |
A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation
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Chapter number | 13 |
Book title |
Acute Myeloid Leukemia
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Published in |
Methods in molecular biology, July 2017
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DOI | 10.1007/978-1-4939-7142-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7140-4, 978-1-4939-7142-8
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Authors |
Ma, Alvin C. H., Shi, Xiangguo, He, Bai-Liang, Guo, Yuhan, Leung, Anskar Y. H., Alvin C. H. Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo, Anskar Y. H. Leung |
Abstract |
The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model. |
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Members of the public | 2 | 100% |