| Chapter title |
Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS
|
|---|---|
| Chapter number | 9 |
| Book title |
Acute Myeloid Leukemia
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7142-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7140-4, 978-1-4939-7142-8
|
| Authors |
Jeong, Mira, Guzman, Anna G., Goodell, Margaret A., Mira Jeong, Anna G. Guzman, Margaret A. Goodell |
| Abstract |
DNA methylation is a major epigenetic modification that regulates gene expression, genome imprinting, and development and has a role in diseases including cancer. There are various methods for whole-genome methylation profiling that differ in cost and resolution. Recent advances in high-throughput sequencing technologies coupled with bisulfite treatment enable absolute DNA methylation quantification and genome-wide single-nucleotide resolution analysis. In this chapter, we provide detailed protocols for whole-genome bisulfite sequencing (WGBS), which captures the complete methylome. Using WGBS, we are able to generate a reference DNA methylome for normal or malignant hematopoietic cells. |
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Demographic breakdown
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Mendeley readers
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| Unspecified | 1 | 7% |
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| Unspecified | 1 | 7% |
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| Other | 0 | 0% |
| Unknown | 5 | 36% |