| Chapter title |
Identifying and Controlling Contamination of Date Palm Tissue Cultures
|
|---|---|
| Chapter number | 14 |
| Book title |
Date Palm Biotechnology Protocols Volume I
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7156-5_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7155-8, 978-1-4939-7156-5
|
| Authors |
Abeer H. I. Abdel-Karim, Abdel-Karim, Abeer H. I. |
| Abstract |
Fungal and bacterial contaminations are major problems facing in vitro date palm (Phoenix dactylifera L.) proliferation. To overcome this problem, we must first identify the fungal (e.g., Alternaria sp., Aspergillus niger, Penicillium sp.) and bacterial (e.g., Pseudomonas sp.) spread in date palm in vitro cultures. Incorporating fungicides (e.g., copper oxychloride, Vitavax T, and Topsin M) or antibiotics (e.g., streptomycin, Banocin, and Bencid D) at 500 mg/L in medium significantly reduces the contamination rate during various stages of in vitro date palm culture. Streptomyces chloramphenicol (pharmacy) is highly effective in reducing the bacterial contamination of date palm cultures to below 10%, as well as enhancing growth vigor. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 22% |
| Student > Master | 2 | 22% |
| Professor | 1 | 11% |
| Lecturer | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 56% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Psychology | 1 | 11% |
| Unknown | 2 | 22% |