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Timeline
Mendeley readers
| Chapter title |
Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts
|
|---|---|
| Chapter number | 17 |
| Book title |
Cell Reprogramming
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2848-4_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2847-7, 978-1-4939-2848-4
|
| Authors |
Christian M. Nefzger, Sara Alaei, Jose M. Polo |
| Abstract |
Mature cells of the body can be reprogrammed towards a pluripotent state by forced expression of the transcription factors Oct-4, Klf-4, Sox2, and C-Myc (OKSM) at very low efficiency. To study the reprogramming process in detail the rare intermediates of the reaction need to be separated from the bulk population. Using a genetically engineered reprogrammable mouse strain we describe how to isolate intermediates from reprogramming cultures of mouse embryonic fibroblasts via antibody labeling of cell surface markers and fluorescence-activated cell sorting (FACS). |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 2 | 29% |
| Student > Ph. D. Student | 2 | 29% |
| Professor > Associate Professor | 2 | 29% |
| Researcher | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 57% |
| Agricultural and Biological Sciences | 2 | 29% |
| Medicine and Dentistry | 1 | 14% |