| Chapter title |
Synthetic mRNA Reprogramming of Human Fibroblast Cells
|
|---|---|
| Chapter number | 2 |
| Book title |
Cell Reprogramming
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2848-4_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2847-7, 978-1-4939-2848-4
|
| Authors |
Jun Liu, Paul J. Verma |
| Abstract |
Reprogramming of somatic cells, such as skin fibroblasts, to pluripotency was first achieved by forced expression of four transcription factors using integrating retroviral or lentiviral vectors, which result in integration of exogenous DNA into cellular genome and present a formidable barrier to therapeutic application of induced pluripotent stem cells (iPSCs). To facilitate the translation of iPSC technology to clinical practice, mRNA reprogramming method that generates transgene-free iPSCs is a safe and efficient method, eliminating bio-containment concerns associated with viral vectors, as well as the need for weeks of screening of cells to confirm that viral material has been completely eliminated during cell passaging. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 22% |
| Student > Ph. D. Student | 3 | 17% |
| Professor | 3 | 17% |
| Student > Master | 2 | 11% |
| Other | 1 | 6% |
| Other | 2 | 11% |
| Unknown | 3 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 33% |
| Neuroscience | 3 | 17% |
| Business, Management and Accounting | 1 | 6% |
| Veterinary Science and Veterinary Medicine | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 28% |