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Timeline
Mendeley readers
| Chapter title |
Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer
|
|---|---|
| Chapter number | 13 |
| Book title |
Cell Reprogramming
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2848-4_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2847-7, 978-1-4939-2848-4
|
| Authors |
Martha C. Gómez, C. Earle Pope |
| Abstract |
In 2003, the first wild felid was produced by interspecies somatic cell nuclear transfer. Since then other wild felid clone offspring have been produced by using the same technique with minor modifications. This chapter describes detailed protocols used in our laboratory for (1) the isolation, culture, and preparation of fibroblast cells as donor nucleus, and (2) embryo reconstruction with domestic cat enucleated oocytes to produce cloned embryos that develop to the blastocyst stage in vitro and, after transfer into synchronized recipients, establish successful pregnancies. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 28% |
| Student > Ph. D. Student | 3 | 17% |
| Student > Master | 2 | 11% |
| Student > Bachelor | 1 | 6% |
| Other | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 4 | 22% |
| Agricultural and Biological Sciences | 4 | 22% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Immunology and Microbiology | 1 | 6% |
| Unknown | 7 | 39% |