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Cell Reprogramming

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Cover of 'Cell Reprogramming'

Table of Contents

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    Book Overview
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    Chapter 1 Cellular Reprogramming in Basic and Applied Biomedicine: The Dawn of Regenerative Medicine.
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    Chapter 2 Synthetic mRNA Reprogramming of Human Fibroblast Cells
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    Chapter 3 MicroRNA-Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells.
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    Chapter 4 Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid Vectors
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    Chapter 5 Reprogramming of Human Fibroblasts with Non-integrating RNA Virus on Feeder-Free or Xeno-Free Conditions
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    Chapter 6 Cell Reprogramming
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    Chapter 7 Generation of Induced Pluripotent Stem Cells (iPSCs) from Adult Canine Fibroblasts.
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    Chapter 8 Cell Reprogramming
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    Chapter 9 Generation of Avian Induced Pluripotent Stem Cells.
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    Chapter 10 Cell Reprogramming
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    Chapter 11 Generation of Efficient Germ-Line Chimeras Using Embryonic Stem Cell Injection
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    Chapter 12 Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation.
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    Chapter 13 Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer
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    Chapter 14 Generation of Chimeras from Porcine Induced Pluripotent Stem Cells
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    Chapter 15 A Novel Method of Somatic Cell Nuclear Transfer with Minimum Equipment
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    Chapter 16 Neonatal Care and Management of Foals Derived by Somatic Cell Nuclear Transfer
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    Chapter 17 Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts
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    Chapter 18 Analysis of Mitochondrial DNA in Induced Pluripotent and Embryonic Stem Cells.
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    Chapter 19 Cell Reprogramming
Attention for Chapter 19: Cell Reprogramming
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Chapter title
Cell Reprogramming
Chapter number 19
Book title
Cell Reprogramming
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2848-4_19
Pubmed ID
26621602
Book ISBNs
978-1-4939-2847-7, 978-1-4939-2848-4
Authors

Taheri-Ghahfarokhi, Amir, Malaver-Ortega, Luis F, Sumer, Huseyin, Malaver-Ortega, Luis F., Amir Taheri-Ghahfarokhi, Luis F. Malaver-Ortega, Huseyin Sumer

Abstract

Interest is increasing in transcription activator-like effector nucleases (TALENs) as a tool to introduce targeted double-strand breaks into the large genomes of human and animal cell lines. The produced DNA lesions stimulate DNA repair pathways, error-prone but dominant non-homologous end joining (NHEJ) and accurate but less occurring homology-directed repair (HDR), and as a result targeted genes can be modified. Here, we describe a modified Golden-Gate cloning method for generating TALENs and also details for targeting genes in mouse embryonic stem cells. The protocol described here can be used for modifying the genome of a broad range of pluripotent cell lines.

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The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 1 100%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 100%

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