Chapter title |
Somatic Embryogenesis and Plant Regeneration of Brachiaria brizantha
|
---|---|
Chapter number | 20 |
Book title |
In Vitro Embryogenesis in Higher Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3061-6_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3060-9, 978-1-4939-3061-6
|
Authors |
Glaucia B. Cabral, Vera T. C. Carneiro, Diva M. A. Dusi, Adriana P. Martinelli |
Abstract |
The genus Brachiaria (Trin.) Griseb. belongs to the family Poaceae, order Poales, class Monocotyledonae. In Brachiaria brizantha (Hochst. ex A. Rich.) Stapf., embryogenic callus can be induced from seeds from apomictic plants, which results in high frequency somatic embryo development and plant regeneration. We report here a detailed protocol for callus induction from apomictic seed; followed by in vitro morphogenesis (somatic embryo and bud differentiation), plant regeneration, and acclimatization in the greenhouse. Important details regarding the positioning of seeds for callus induction and precautions to avoid endophytic contamination and the occurrence of albino plants are presented. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 21% |
Researcher | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Student > Bachelor | 1 | 7% |
Professor | 1 | 7% |
Other | 1 | 7% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 50% |
Biochemistry, Genetics and Molecular Biology | 3 | 21% |
Unknown | 4 | 29% |