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Mendeley readers
Chapter title |
Somatic Embryogenesis in Horse Chestnut ( Aesculus hippocastanum L.)
|
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Chapter number | 23 |
Book title |
In Vitro Embryogenesis in Higher Plants
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3061-6_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3060-9, 978-1-4939-3061-6
|
Authors |
Maurizio Capuana |
Abstract |
Embryogenic cultures of horse chestnut (Aesculus hippocastanum L.) can be obtained from different organs and tissues. We describe here the induction from stamen filaments and the procedures applied for the successive phases of somatic embryo development and maturation. Embryogenic tissues are obtained on Murashige and Skoog medium containing 9.0 μM 2,4-dichlorophenoxyacetic acid. Somatic embryos develop after transfer to hormone-free medium enriched with glutamine. Maturation and germination of isolated embryos are achieved by transfer to medium containing polyethylene glycol 4000 and activated charcoal, successive desiccation treatment, and cold storage at 4 °C for 8 weeks. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 50% |
Unknown | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 50% |
Unknown | 1 | 50% |