Chapter title |
Somatic Embryogenesis in Olive ( Olea europaea L. subsp. europaea var. sativa and var. sylvestris )
|
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Chapter number | 15 |
Book title |
In Vitro Embryogenesis in Higher Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3061-6_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3060-9, 978-1-4939-3061-6
|
Authors |
Eddo Rugini, Cristian Silvestri |
Abstract |
Protocols for olive somatic embryogenesis from zygotic embryos and mature tissues have been described for both Olea europaea sub. europaea var. sativa and var. sylvestris. Immature zygotic embryos (no more than 75 days old), used after fruit collection or stored at 12-14 °C for 2-3 months, are the best responsive explants and very slightly genotype dependent, and one single protocol can be effective for a wide range of genotypes. On the contrary, protocols for mature zygotic embryos and for mature tissue of cultivars are often genotype specific, so that they may require many adjustments according to genotypes. The use of thidiazuron and cefotaxime seems to be an important trigger for induction phase particularly for tissues derived from cultivars. Up to now, however, the application of this technique for large-scale propagation is hampered also by the low rate of embryo germination; it proves nonetheless very useful for genetic improvement. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 22% |
Student > Bachelor | 2 | 11% |
Professor | 2 | 11% |
Student > Doctoral Student | 1 | 6% |
Researcher | 1 | 6% |
Other | 1 | 6% |
Unknown | 7 | 39% |
Readers by discipline | Count | As % |
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Chemical Engineering | 1 | 6% |
Unknown | 8 | 44% |