Chapter title |
Somatic Embryogenesis in Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae)
|
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Chapter number | 24 |
Book title |
In Vitro Embryogenesis in Higher Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3061-6_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3060-9, 978-1-4939-3061-6
|
Authors |
Miguel P. Guerra, Neusa Steiner, Francine L. Farias-Soares, Leila do N. Vieira, Hugo P. F. Fraga, Gladys D. Rogge-Renner, Sara B. Maldonado |
Abstract |
This chapter deals with the features of somatic embryogenesis (SE) in Araucaria angustifolia, an endangered and native conifer from south Brazil. In this species SE includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (PEMs), which precede somatic embryos development. A. angustifolia SE model encompasses induction, proliferation, pre-maturation, and maturation steps. Double-staining with acetocarmine and Evan's blue is useful to evaluate the embryonic somatic structures. In this chapter we describe A. angustifolia SE protocols and analyzes morphological features in the different SE developmental stages. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 25% |
Professor | 2 | 25% |
Other | 1 | 13% |
Student > Bachelor | 1 | 13% |
Student > Master | 1 | 13% |
Other | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 88% |
Unknown | 1 | 13% |