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Mendeley readers
Chapter title |
Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors
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Chapter number | 15 |
Book title |
Natural Antibodies
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Published in |
Methods in molecular biology, July 2017
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DOI | 10.1007/978-1-4939-7180-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7179-4, 978-1-4939-7180-0
|
Authors |
Zhili Xu, Jie Tian, Andrew W. Harmon, Andrew P. Byrnes |
Editors |
Srinivas V. Kaveri, Jagadeesh Bayry |
Abstract |
Natural IgM antibodies have an innate ability to recognize many viruses and viral-based gene therapy vectors. Naive mice have natural IgM antibodies that bind to adenoviruses, and these antibodies can profoundly affect the biodistribution and efficiency of gene delivery by adenovirus type 5 vectors. Here, we present protocols for isolating IgM from mouse serum, for assaying the concentration and adenoviral reactivity of mouse IgM, and for evaluating how natural antibodies and complement can synergize to neutralize adenovirus vectors. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 40% |
Researcher | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Unknown | 4 | 80% |