Chapter title |
Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid
|
---|---|
Chapter number | 3 |
Book title |
Natural Antibodies
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7180-0_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7179-4, 978-1-4939-7180-0
|
Authors |
Christoph Schneider Ph.D., Marlies Illi, Marius Lötscher Ph.D., Marc Wehrli M.D., Ph.D., Stephan von Gunten M.D., Ph.D., M.M.E, Christoph Schneider, Marius Lötscher, Marc Wehrli, Stephan von Gunten, Schneider, Christoph, Illi, Marlies, Lötscher, Marius, Wehrli, Marc, Gunten, Stephan |
Editors |
Srinivas V. Kaveri, Jagadeesh Bayry |
Abstract |
Different protocols are required for the collection and isolation of antibodies from various body sites. For the sample collection factors to be considered include anatomic or physiological particularities. Secretory fluids such as saliva, gastrointestinal fluid, or breast milk may contain degrading enzymes that potentially affect the integrity of isolated antibodies. While the isolation of IgG from plasma is a common and often-described procedure, here we focus on methodological approaches to isolate antibodies immunoglobulin A (IgA) or IgM from plasma or secretory fluids. These protocols shall facilitate research on natural and induced antibodies. |
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