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Natural Antibodies

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Cover of 'Natural Antibodies'

Table of Contents

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    Book Overview
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    Chapter 1 Natural Antibodies: Next Steps Toward Translational Investigation
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    Chapter 2 Isolation of Natural Anti-FcεRIα Autoantibodies from Healthy Donors
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    Chapter 3 Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid
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    Chapter 4 Purification of Natural Antibodies Against Tau Protein by Affinity Chromatography
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    Chapter 5 Unbiased RACE-Based Massive Parallel Surveys of Human IgA Antibody Repertoires
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    Chapter 6 Analysis of Signaling Events in B-1a Cells
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    Chapter 7 Exploring the Role of Microbiota in the Limiting of B1 and MZ B-Cell Numbers by Naturally Secreted Immunoglobulins
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    Chapter 8 Assessment of Anti-Tumor Cytotoxic Activity of Naturally Occurring Antibodies in Human Serum or Plasma
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    Chapter 9 Hydrolysis and Dissolution of Amyloids by Catabodies
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    Chapter 10 Methods for Posttranslational Induction of Polyreactivity of Antibodies
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    Chapter 11 Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles
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    Chapter 12 Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans
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    Chapter 13 Detection of Natural Antibodies and Serological Diagnosis of Pneumococcal Pneumonia Using a Bead-Based High-Throughput Assay
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    Chapter 14 Detection of Naturally Occurring Human Antibodies Against Gangliosides by ELISA
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    Chapter 15 Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors
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    Chapter 16 Erratum to: Hydrolysis and Dissolution of Amyloids by Catabodies
Attention for Chapter 6: Analysis of Signaling Events in B-1a Cells
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Chapter title
Analysis of Signaling Events in B-1a Cells
Chapter number 6
Book title
Natural Antibodies
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7180-0_6
Pubmed ID
Book ISBNs
978-1-4939-7179-4, 978-1-4939-7180-0
Authors

Jianxin Huo, Yuhan Huang, Shengli Xu, Kong-Peng Lam

Editors

Srinivas V. Kaveri, Jagadeesh Bayry

Abstract

B-1a cells are a unique subset of B cells that are phenotypically and functionally distinguishable from conventional (B-2) cells. Here we detail methods to analyze signaling events in B-1a cells. We demonstrate by flow cytometry and Western blot that mouse peritoneal B-1a but not splenic B-2 cells have constitutive activation of signal transducers and activators of transcription-3 (Stat3) and extracellular signal-regulated kinase (Erk).

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Professor > Associate Professor 1 33%
Researcher 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Unknown 2 67%