| Chapter title |
Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans
|
|---|---|
| Chapter number | 12 |
| Book title |
Natural Antibodies
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7180-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7179-4, 978-1-4939-7180-0
|
| Authors |
Chunguang Wang, Sohvi Hörkkö, Wang, Chunguang, Hörkkö, Sohvi |
| Editors |
Srinivas V. Kaveri, Jagadeesh Bayry |
| Abstract |
Natural antibodies are produced by B lymphocytes without exogenous antigenic exposure and are present at the time of birth. They usually bind to conserved epitopes on antigens of different chemical compositions. We cloned and characterized a natural mouse monoclonal IgM antibody (Aa_Mab) by selecting the binding to malondialdehyde acetaldehyde (MAA) adducts on low-density lipoprotein (LDL). The data showed that the Aa_Mab cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, an important oral pathogen in periodontitis associated with atherosclerosis. Surprisingly, the binding molecule of Aa bacteria to the Aa_Mab was Aa chaperonin 60 or HSP60, a protein that is not only responsible for maintaining cellular proteins conformation, but also functions as a potent virulence factor prompting bone resorption in periodontitis and as a putative pathogenic factor in atherosclerosis. |
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|---|---|---|
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