| Chapter title |
Mass Spectrometry-Based Biomarker Discovery
|
|---|---|
| Chapter number | 19 |
| Book title |
Molecular Profiling
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6990-6_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6989-0, 978-1-4939-6990-6
|
| Authors |
Weidong Zhou Ph.D., Emanuel F. Petricoin III Ph.D., Caterina Longo M.D., Weidong Zhou, Emanuel F. Petricoin, Caterina Longo |
| Editors |
Virginia Espina |
| Abstract |
The discovery of candidate biomarkers within the entire proteome is one of the most important and challenging goals in proteomic research. Mass spectrometry-based proteomics is a modern and promising technology for semiquantitative and qualitative assessment of proteins, enabling protein sequencing and identification with exquisite accuracy and sensitivity. For mass spectrometry analysis, protein extractions from tissues or body fluids and subsequent protein fractionation represent an important and unavoidable step in the workflow for biomarker discovery. Following extraction of proteins, the protein mixture must be digested, reduced, alkylated, and cleaned up prior to mass spectrometry. The aim of our chapter is to provide comprehensible and practical lab procedures for sample digestion, protein fractionation, and subsequent mass spectrometry analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 16% |
| Researcher | 4 | 11% |
| Professor | 4 | 11% |
| Student > Ph. D. Student | 3 | 8% |
| Student > Bachelor | 2 | 5% |
| Other | 9 | 24% |
| Unknown | 10 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 16% |
| Biochemistry, Genetics and Molecular Biology | 3 | 8% |
| Agricultural and Biological Sciences | 3 | 8% |
| Chemistry | 3 | 8% |
| Immunology and Microbiology | 2 | 5% |
| Other | 8 | 21% |
| Unknown | 13 | 34% |