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Chapter title |
Optimization of Immunostaining for Prospective Image Analysis
|
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Chapter number | 16 |
Book title |
Molecular Profiling
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6990-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6989-0, 978-1-4939-6990-6
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Authors |
Scott M. Lawrence M.S., H.T. (A.S.C.P.), Yelena G. Golubeva Ph.D., H.T. (A.S.C.P.), Scott M. Lawrence, Yelena G. Golubeva |
Editors |
Virginia Espina |
Abstract |
Biomarker discovery is a crucial part of the fast developing field of personalized medicine. Antibody-based techniques including immunostaining of tissue samples are widely used for biomarker evaluation in preclinical and clinical studies. When used in conjunction with robust image analysis methods, it provides a powerful means to assess biomarker modulation, toxicity, and patient response to targeted agents. Here, we describe the optimization of immunofluorescent (IF) staining protocols and a sample IF multiplex protocol suitable for colocalization image analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 1 | 33% |
Student > Bachelor | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Medicine and Dentistry | 1 | 33% |