Chapter title |
Scanning Electron Microscopy Sample Preparation and Imaging
|
---|---|
Chapter number | 5 |
Book title |
Molecular Profiling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6990-6_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6989-0, 978-1-4939-6990-6
|
Authors |
Jenny Ngoc Tran Nguyen, Amanda M. Harbison |
Editors |
Virginia Espina |
Abstract |
Scanning electron microscopes allow us to reach magnifications of 20-130,000× and resolve compositional and topographical images with intense detail. These images are created by bombarding a sample with electrons in a focused manner to generate a black and white image from the electrons that bounce off of the sample. The electrons are detected using positively charged detectors. Scanning electron microscopy permits three-dimensional imaging of desiccated specimens or wet cells and tissues by using variable pressure chambers. SEM ultrastructural analysis and intracellular imaging supplement light microscopy for molecular profiling of prokaryotes, plants, and mammals. This chapter demonstrates how to prepare and image samples that are (a) desiccated and conductive, (b) desiccated and nonconductive but coated with an electron conductive film using a gold sputter coater, and |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 100 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 17 | 17% |
Student > Master | 11 | 11% |
Student > Ph. D. Student | 9 | 9% |
Student > Doctoral Student | 3 | 3% |
Student > Postgraduate | 3 | 3% |
Other | 8 | 8% |
Unknown | 49 | 49% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 11 | 11% |
Chemistry | 7 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 6% |
Biochemistry, Genetics and Molecular Biology | 6 | 6% |
Materials Science | 5 | 5% |
Other | 15 | 15% |
Unknown | 50 | 50% |