Chapter title |
PCR: Identification of Genetic Polymorphisms
|
---|---|
Chapter number | 13 |
Book title |
Molecular Profiling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6990-6_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6989-0, 978-1-4939-6990-6, 978-1-4939-6989-0, 978-1-4939-6990-6
|
Authors |
Amanda M. Harbison, Jenny Ngoc Tran Nguyen |
Editors |
Virginia Espina |
Abstract |
Polymerase chain reaction (PCR) enables the amplification of a specific sequence of deoxyribonucleic acid (DNA) through the process of three main steps: template DNA denaturation, annealing of the primers to complementary sequences, and primer extension to synthesize DNA strands. By using this method, the target sequence will be copied and amplified at an exponential rate. PCR provides a qualitative method for identifying DNA from fresh or dried cells/body fluids, formalin-fixed archival tissue specimens, and ancient specimens.Herein we describe basic information for performing successful PCR experiments using the amplification of a human Alu insertion on the PV92 gene locus on chromosome 16 as an example method. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 57 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 23 | 40% |
Student > Master | 7 | 12% |
Student > Postgraduate | 2 | 4% |
Student > Doctoral Student | 2 | 4% |
Unspecified | 1 | 2% |
Other | 2 | 4% |
Unknown | 20 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 16 | 28% |
Agricultural and Biological Sciences | 6 | 11% |
Medicine and Dentistry | 5 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
Nursing and Health Professions | 2 | 4% |
Other | 2 | 4% |
Unknown | 23 | 40% |