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The Bacterial Flagellum

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Cover of 'The Bacterial Flagellum'

Table of Contents

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    Book Overview
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    Chapter 1 Fuel of the Bacterial Flagellar Type III Protein Export Apparatus
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    Chapter 2 Interactions of Flagellar Structural Subunits with the Membrane Export Machinery
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    Chapter 3 Fluorescent Microscopy Techniques to Study Hook Length Control and Flagella Formation
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    Chapter 4 Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica
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    Chapter 5 Dynamic Measures of Flagellar Gene Expression
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    Chapter 6 Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica
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    Chapter 7 Design and Preparation of the Fragment Proteins of the Flagellar Components Suitable for X-Ray Crystal Structure Analysis
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    Chapter 8 Structural Analysis of the Flagellar Component Proteins in Solution by Small Angle X-Ray Scattering
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    Chapter 9 Structural Study of the Bacterial Flagellar Basal Body by Electron Cryomicroscopy and Image Analysis
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    Chapter 10 Structure of the MotA/B Proton Channel
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    Chapter 11 Mechanism of Stator Assembly and Incorporation into the Flagellar Motor
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    Chapter 12 Rotation Measurements of Tethered Cells
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    Chapter 13 Tracking the Movement of a Single Prokaryotic Cell in Extreme Environmental Conditions
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    Chapter 14 Measurements of the Rotation of the Flagellar Motor by Bead Assay
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    Chapter 15 Measurements of Ion-Motive Force Across the Cell Membrane
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    Chapter 16 Stoichiometry and Turnover of the Stator and Rotor
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    Chapter 17 Direct Imaging of Intracellular Signaling Molecule Responsible for the Bacterial Chemotaxis
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    Chapter 18 In Situ Structural Analysis of the Spirochetal Flagellar Motor by Cryo-Electron Tomography
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    Chapter 19 Motility of Spirochetes
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    Chapter 20 Structure of the Sodium-Driven Flagellar Motor in Marine Vibrio
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    Chapter 21 Chemotactic Behaviors of Vibrio cholerae Cells
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    Chapter 22 Purification of Fla2 Flagella of Rhodobacter sphaeroides
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    Chapter 23 Dynamics in the Dual Fuel Flagellar Motor of Shewanella oneidensis MR-1
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    Chapter 24 Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species
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    Chapter 25 Measurement of Free-Swimming Motility and Magnetotactic Behavior of Magnetococcus massalia Strain MO-1
Attention for Chapter 24: Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species
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Chapter title
Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species
Chapter number 24
Book title
The Bacterial Flagellum
Published in
Methods in molecular biology, April 2017
DOI 10.1007/978-1-4939-6927-2_24
Pubmed ID
Book ISBNs
978-1-4939-6926-5, 978-1-4939-6927-2
Authors

Yuka Takahashi, Masahiro Ito

Editors

Tohru Minamino, Keiichi Namba

Abstract

Many bacteria can swim using their flagella, which are filamentous organelles that extend from the cell surface. The flagellar motor is energized by either a proton (H(+)) or sodium ion (Na(+)) as the motive force. MotAB-type stators use protons, whereas MotPS- and PomAB-type stators use Na(+) as the coupling ions. Recently, alkaliphilic Bacillus alcalophilus was shown to use potassium ions (K(+)) and rubidium ions (Rb(+)) for flagellar rotation, and the flagellar motor from Paenibacillus sp. TCA-20 uses divalent cations such as magnesium ions (Mg(2+)), calcium ions (Ca(2+)), and strontium ions (Sr(2+)) for coupling. In this chapter, we focus on how to identify the coupling ions for flagellar rotation of alkaliphilic Bacillus and Paenibacillus species.

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Researcher 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Sports and Recreations 1 25%
Unknown 1 25%