| Chapter title |
Structure of the Sodium-Driven Flagellar Motor in Marine Vibrio
|
|---|---|
| Chapter number | 20 |
| Book title |
The Bacterial Flagellum
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6927-2_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6926-5, 978-1-4939-6927-2
|
| Authors |
Yasuhiro Onoue, Michio Homma |
| Editors |
Tohru Minamino, Keiichi Namba |
| Abstract |
Most bacteria can swim by rotating the flagellum. The basal body of the flagellum is an essential part for this motor function. Recent comprehensive analysis of the flagellar basal body structures across bacteria by cryo-electron tomography has revealed that they all share core structures, the rod, and rings: the C ring, M ring, S ring, L ring, and P ring. Furthermore, it also has uncovered that in some bacteria, there are extra ring structures in the periplasmic space and outer-membrane. Here, we describe a protocol to isolate the basal body of the flagellar basal body from a marine bacterium, Vibrio alginolyticus, for structural analysis of additional ring structures, the T ring and H ring. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 50% |
| Unknown | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 1 | 50% |
| Unknown | 1 | 50% |