Chapter title |
Tracking the Movement of a Single Prokaryotic Cell in Extreme Environmental Conditions
|
---|---|
Chapter number | 13 |
Book title |
The Bacterial Flagellum
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6927-2_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6926-5, 978-1-4939-6927-2
|
Authors |
Nishiyama, Masayoshi, Arai, Yoshiyuki, Masayoshi Nishiyama, Yoshiyuki Arai |
Editors |
Tohru Minamino, Keiichi Namba |
Abstract |
Many bacterial species move toward favorable habitats. The flagellum is one of the most important machines required for the motility in solution and is conserved across a wide range of bacteria. The motility machinery is thought to function efficiently with a similar mechanism in a variety of environmental conditions, as many cells with similar machineries have been isolated from harsh environments. To understand the common mechanism and its diversity, microscopic examination of bacterial movements is a crucial step. Here, we describe a method to characterize the swimming motility of cells in extreme environmental conditions. This microscopy system enables acquisition of high-resolution images under high-pressure conditions. The temperature and oxygen concentration can also be manipulated. In addition, we also describe a method to track the movement of swimming cells using an ImageJ plugin. This enables characterization of the swimming motility of the selected cells. |
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