Chapter title |
Purification of Fla2 Flagella of Rhodobacter sphaeroides
|
---|---|
Chapter number | 22 |
Book title |
The Bacterial Flagellum
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6927-2_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6926-5, 978-1-4939-6927-2
|
Authors |
Javier de la Mora, Laura Camarena, Georges Dreyfus |
Editors |
Tohru Minamino, Keiichi Namba |
Abstract |
The photosynthetic bacterium R. sphaeroides expresses two flagellar systems that are encoded by two complete gene clusters that have distinct phylogenetic origins. The isolation and purification of the Filament-Hook Basal Body (F-HBB) or the Hook Basal Body (HBB) structure is a troublesome task given the complexity of this nano-machine that is composed of multiple loosely bound substructures that can be lost during the isolation and purification procedure. A successful procedure requires adjustments to the standard method established for Salmonella. In this chapter, we describe a detailed protocol to isolate and purify the Fla2 F-HBB and HBB from R. sphaeroides a photosynthetic bacterium that has a complex intracellular membrane system that frequently interferes with isolation of high-quality samples. |
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