Chapter title |
Interactions of Flagellar Structural Subunits with the Membrane Export Machinery
|
---|---|
Chapter number | 2 |
Book title |
The Bacterial Flagellum
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6927-2_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6926-5, 978-1-4939-6927-2
|
Authors |
Evans, Lewis D. B., Bergen, Paul M., Bryant, Owain J., Fraser, Gillian M., Lewis D. B. Evans, Paul M. Bergen, Owain J. Bryant, Gillian M. Fraser |
Editors |
Tohru Minamino, Keiichi Namba |
Abstract |
During assembly of the bacterial flagellum, structural subunits synthesized inside the cell must be exported across the cytoplasmic membrane before they can crystallize into the nascent flagellar structure. This export process is facilitated by a specialized Flagellar Type III Secretion System (fT3SS) located at the base of each flagellum. Here, we describe three methods-isothermal titration calorimetry, photo-crosslinking using unnatural amino acids, and a subunit capture assay-used to investigate the interactions of flagellar structural subunits with the membrane export machinery component FlhB. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 25% |
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 50% |
Computer Science | 1 | 25% |
Medicine and Dentistry | 1 | 25% |