Chapter title |
Microfluidic “Pouch” Chips for Immunoassays and Nucleic Acid Amplification Tests
|
---|---|
Chapter number | 30 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Michael G. Mauk, Changchun Liu, Xianbo Qiu, Dafeng Chen, Jinzhao Song, Haim H. Bau, Mauk, Michael G., Liu, Changchun, Qiu, Xianbo, Chen, Dafeng, Song, Jinzhao, Bau, Haim H. |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
Microfluidic cassettes ("chips") for processing and analysis of clinical specimens and other sample types facilitate point-of-care (POC) immunoassays and nucleic acid based amplification tests. These single-use test chips can be self-contained and made amenable to autonomous operation-reducing or eliminating supporting instrumentation-by incorporating laminated, pliable "pouch" and membrane structures for fluid storage, pumping, mixing, and flow control. Materials and methods for integrating flexible pouch compartments and diaphragm valves into hard plastic (e.g., acrylic and polycarbonate) microfluidic "chips" for reagent storage, fluid actuation, and flow control are described. We review several versions of these pouch chips for immunoassay and nucleic acid amplification tests, and describe related fabrication techniques. These protocols thus offer a "toolbox" of methods for storage, pumping, and flow control functions in microfluidic devices. |
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