Chapter title |
Extended Gate Field-Effect Transistor Biosensors for Point-Of-Care Testing of Uric Acid
|
---|---|
Chapter number | 13 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Weihua Guan, Mark A. Reed |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
An enzyme-free redox potential sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode has been used to quantify uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. The potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific to uric acid in human serum and urine. The interference that comes from glucose, bilirubin, ascorbic acid, and hemoglobin is negligible in the normal concentration range of these interferents. The sensor also exhibits excellent long term reliability and is regenerative. This extended gate field effect transistor based sensor is promising for point-of-care detection of uric acid due to the small size, low cost, and low sample volume consumption. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 31% |
Professor | 2 | 15% |
Student > Bachelor | 2 | 15% |
Unspecified | 1 | 8% |
Other | 1 | 8% |
Other | 2 | 15% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 3 | 23% |
Medicine and Dentistry | 2 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Unspecified | 1 | 8% |
Other | 3 | 23% |
Unknown | 2 | 15% |