Chapter title |
Microfluidic Arrayed Lab-On-A-Chip for Electrochemical Capacitive Detection of DNA Hybridization Events
|
---|---|
Chapter number | 6 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Hadar Ben-Yoav, Peter H. Dykstra, William E. Bentley, Reza Ghodssi |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
A microfluidic electrochemical lab-on-a-chip (LOC) device for DNA hybridization detection has been developed. The device comprises a 3 × 3 array of microelectrodes integrated with a dual layer microfluidic valved manipulation system that provides controlled and automated capabilities for high throughput analysis of microliter volume samples. The surface of the microelectrodes is functionalized with single-stranded DNA (ssDNA) probes which enable specific detection of complementary ssDNA targets. These targets are detected by a capacitive technique which measures dielectric variation at the microelectrode-electrolyte interface due to DNA hybridization events. A quantitative analysis of the hybridization events is carried out based on a sensing modeling that includes detailed analysis of energy storage and dissipation components. By calculating these components during hybridization events the device is able to demonstrate specific and dose response sensing characteristics. The developed microfluidic LOC for DNA hybridization detection offers a technology for real-time and label-free assessment of genetic markers outside of laboratory settings, such as at the point-of-care or in-field environmental monitoring. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 47% |
Unspecified | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Lecturer > Senior Lecturer | 1 | 7% |
Professor | 1 | 7% |
Other | 1 | 7% |
Unknown | 3 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 20% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Engineering | 2 | 13% |
Physics and Astronomy | 1 | 7% |
Unspecified | 1 | 7% |
Other | 2 | 13% |
Unknown | 4 | 27% |