Chapter title |
Piezoelectric Cantilever Biosensors for Label-free, Real-time Detection of DNA and RNA
|
---|---|
Chapter number | 17 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Alexander P. Haring, Ellen Cesewski, Blake N. Johnson |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
This chapter reviews the design, fabrication, characterization, and application of piezoelectric-excited millimeter-sized cantilever (PEMC) sensors. The sensor transduction mechanism, sensing principle, and mode of operation are discussed. Bio-recognition strategies and surface functionalization methods for detection of DNA and RNA are discussed with a focus on self-assembly-based approaches. Methods for the verification of biosensor response via secondary binding assays, reversible binding assays, and the integration of complementary transduction mechanisms are presented. Sensing applications for medical diagnostics, food safety, and environmental monitoring are provided. PEMC sensor technology provides a robust platform for the real-time, label-free detection of DNA and RNA in complex matrices over nanomolar (nM) to attomolar (aM) concentration ranges. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 23% |
Unspecified | 2 | 15% |
Researcher | 2 | 15% |
Student > Master | 2 | 15% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 5 | 38% |
Unspecified | 2 | 15% |
Agricultural and Biological Sciences | 2 | 15% |
Medicine and Dentistry | 1 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Other | 0 | 0% |
Unknown | 2 | 15% |