Chapter title |
Bacterial Detection Using Peptide-Based Platform and Impedance Spectroscopy
|
---|---|
Chapter number | 8 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Hashem Etayash, Thomas Thundat, Kamaljit Kaur |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
Antimicrobial peptides have the ability to function as bio-recognition elements in the detection of bacteria. For instance, we showed that Leucocin A, an antimicrobial peptide from class IIa bacteriocins, binds gram-positive Listeria monocytogenes with higher affinity than other gram-positive bacteria like S. aureus, L. innocua, and E. faecalis. The binding was detected using impedance spectroscopy when Leucocin A immobilized on impedance electrodes binds bacteria from a sample. Here we highlight the strength of utilizing Leucocin A as a bio-recognition probe in biosensor platforms and provide details on its application in real-time bacterial detection using electrochemical impedance spectroscopy. A simple new generation impedance array analyzer is utilized that works at very low frequencies to identify interactions between peptide and the target bacteria. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 25% |
Professor > Associate Professor | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Chemistry | 1 | 25% |
Unknown | 1 | 25% |