Chapter title |
Synthetic Cell-Based Sensors with Programmed Selectivity and Sensitivity
|
---|---|
Chapter number | 23 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6911-1_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6910-4, 978-1-4939-6911-1
|
Authors |
Elvis Bernard, Baojun Wang |
Editors |
Ben Prickril, Avraham Rasooly |
Abstract |
Bacteria live in an ever changing environment and, to adapt their physiology, they have to sense the changes. Our current understanding of the mechanisms and elements involved in the detection and processing of these environmental signals grant us access to an array of genetic components able to process such information. As engineers can use different electronic components to build a circuit, we can rewire the cellular components to create digital logic and analogue gene circuits that will program cell behaviour in a designed manner in response to a specific stimulus. Here we present the methods and protocols for designing and implementing synthetic cell-based biosensors that use engineered genetic logic and analogue amplifying circuits to significantly increase selectivity and sensitivity, for example, for heavy metal ions in an aqueous environment. The approach is modular and can be readily applied to improving the sensing limit and performance of a range of microbial cell-based sensors to meet their real world detection requirement. |
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Student > Master | 5 | 26% |
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Lecturer | 1 | 5% |
Researcher | 1 | 5% |
Other | 0 | 0% |
Unknown | 5 | 26% |
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Biochemistry, Genetics and Molecular Biology | 5 | 26% |
Unspecified | 1 | 5% |
Engineering | 1 | 5% |
Unknown | 6 | 32% |