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Mendeley readers
Chapter title |
Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells
|
---|---|
Chapter number | 5 |
Book title |
Thermogenic Fat
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Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6820-6_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6819-0, 978-1-4939-6820-6
|
Authors |
Rohollah Babaei, Irem Bayindir-Buchhalter, Irina Meln, Alexandros Vegiopoulos |
Editors |
Jun Wu |
Abstract |
Appropriate cell models are necessary for the investigation of thermogenic beige adipocyte differentiation from progenitor cells. Here, we describe a primary cell culture method that is based on defined progenitor cells from murine white adipose tissue and aims at minimizing confounding factors including cell heterogeneity and nonphysiological differentiation inducers. Adipocyte progenitor cells are enriched by immuno-magnetic separation, expanded minimally, and induced for beige adipocyte differentiation with carbaprostacyclin, a stable analogue of the endogenous mediator PGI2. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 50% |
Student > Ph. D. Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 50% |
Unknown | 2 | 50% |