| Chapter title |
In Vitro Approaches to Model and Study Communication Between Adipose Tissue and the Liver
|
|---|---|
| Chapter number | 15 |
| Book title |
Thermogenic Fat
|
| Published in |
Methods in molecular biology, February 2017
|
| DOI | 10.1007/978-1-4939-6820-6_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6819-0, 978-1-4939-6820-6
|
| Authors |
Sean O’Connor, Paul Cohen |
| Editors |
Jun Wu |
| Abstract |
Inter-organ communication is central to mammalian metabolism, allowing for the coordination of crucial homeostatic processes such as food intake, body weight, and blood glucose. Dissecting this process at a cellular and molecular level requires the establishment of a reductionist system in which cell types of interest can be investigated as the source and target of biologically relevant signals. Here, we describe a system to study the interaction between adipocytes and hepatocytes using either conditioned media from cultured adipocytes or co-culture of hepatocytes with adipose explants. These methods have the potential to identify novel polypeptides and metabolites involved in the adipose-liver metabolic axis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 20% |
| Student > Bachelor | 2 | 20% |
| Researcher | 2 | 20% |
| Student > Postgraduate | 2 | 20% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 50% |
| Agricultural and Biological Sciences | 2 | 20% |
| Engineering | 1 | 10% |
| Unknown | 2 | 20% |