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Thermogenic Fat

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Cover of 'Thermogenic Fat'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue
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    Chapter 2 Isolation of Mouse Stromal Vascular Cells for Monolayer Culture
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    Chapter 3 Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells
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    Chapter 4 Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes
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    Chapter 5 Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells
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    Chapter 6 Isolation of Immune Cells from Adipose Tissue for Flow Cytometry
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    Chapter 7 Differentiation and Metabolic Interrogation of Human Adipocytes
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    Chapter 8 Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines
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    Chapter 9 Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue
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    Chapter 10 Genetic Mouse Models: The Powerful Tools to Study Fat Tissues
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    Chapter 11 Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
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    Chapter 12 Bioenergetic Analyses in Adipose Tissue
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    Chapter 13 Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry
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    Chapter 14 Isolation and Patch-Clamp of Primary Adipocytes
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    Chapter 15 In Vitro Approaches to Model and Study Communication Between Adipose Tissue and the Liver
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    Chapter 16 Identification and Quantification of Human Brown Adipose Tissue
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    Chapter 17 Designing 3-D Adipospheres for Quantitative Metabolic Study
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    Chapter 18 Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems
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    Chapter 19 Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides
Attention for Chapter 11: Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
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Chapter title
Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
Chapter number 11
Book title
Thermogenic Fat
Published in
Methods in molecular biology, February 2017
DOI 10.1007/978-1-4939-6820-6_11
Pubmed ID
Book ISBNs
978-1-4939-6819-0, 978-1-4939-6820-6
Authors

Nicolás Gómez-Banoy, James C. Lo

Editors

Jun Wu

Abstract

Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 40%
Student > Bachelor 1 20%
Professor > Associate Professor 1 20%
Unknown 1 20%
Readers by discipline Count As %
Medicine and Dentistry 2 40%
Neuroscience 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%
Unknown 1 20%