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Mendeley readers
Chapter title |
Isolation and Patch-Clamp of Primary Adipocytes
|
---|---|
Chapter number | 14 |
Book title |
Thermogenic Fat
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6820-6_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6819-0, 978-1-4939-6820-6
|
Authors |
Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, Isaac Samuel, Jessica K. Smith, Rajan Sah |
Editors |
Jun Wu |
Abstract |
The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature adipocytes from both mouse and human white adipose tissues (subcutaneous and visceral). From the same preparation, we describe how to culture and differentiate preadipocytes isolated from the stromal vascular fraction. We then describe in detail patch-clamp methods, including both whole-cell and perforated-patch configurations. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 38% |
Researcher | 2 | 15% |
Student > Doctoral Student | 1 | 8% |
Unspecified | 1 | 8% |
Professor | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 31% |
Unspecified | 1 | 8% |
Environmental Science | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Physics and Astronomy | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 31% |