Chapter title |
Designing 3-D Adipospheres for Quantitative Metabolic Study
|
---|---|
Chapter number | 17 |
Book title |
Thermogenic Fat
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6820-6_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6819-0, 978-1-4939-6820-6
|
Authors |
Takeshi Akama, Brendan M. Leung, Joe Labuz, Shuichi Takayama, Tae-Hwa Chun, Akama, Takeshi, Leung, Brendan M., Labuz, Joe, Takayama, Shuichi, Chun, Tae-Hwa |
Editors |
Jun Wu |
Abstract |
Quantitative assessment of adipose mitochondrial activity is critical for better understanding of adipose tissue function in obesity and diabetes. While the two-dimensional (2-D) tissue culture method has been sufficient to discover key molecules that regulate adipocyte differentiation and function, the method is insufficient to determine the role of extracellular matrix (ECM) molecules and their modifiers, such as matrix metalloproteinases (MMPs), in regulating adipocyte function in three-dimensional (3-D) in vivo-like microenvironments. By using a 3-D hanging drop tissue culture system, we are able to produce scalable 3-D adipospheres that are suitable for quantitative metabolic study in 3-D microenvironment. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 19% |
Student > Ph. D. Student | 3 | 19% |
Student > Master | 2 | 13% |
Student > Doctoral Student | 1 | 6% |
Other | 1 | 6% |
Other | 2 | 13% |
Unknown | 4 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 38% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Engineering | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 38% |