| Chapter title |
Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems
|
|---|---|
| Chapter number | 18 |
| Book title |
Thermogenic Fat
|
| Published in |
Methods in molecular biology, February 2017
|
| DOI | 10.1007/978-1-4939-6820-6_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6819-0, 978-1-4939-6820-6
|
| Authors |
Brooks, Jessica C., Judd, Robert L., Easley, Christopher J., Jessica C. Brooks, Robert L. Judd, Christopher J. Easley |
| Editors |
Jun Wu |
| Abstract |
Microfluidic culture of primary adipose tissue allows for reduced sample and reagent volumes as well as constant media perfusion of the cells. By continuously flowing media over the tissue, microfluidic sampling systems can more accurately mimic vascular flow in vivo. Quantitative measurements can be performed on or off chip to provide time-resolved secretion data, furthering insight into the dynamics of the function of adipose tissue. Buoyancy resulting from the large lipid storage capacity in this tissue presents a unique challenge for culture, and it is important to account for this buoyancy during microdevice design. Herein, we describe approaches for microfluidic device fabrication that utilize 3D-printed interface templating to help counteract cell buoyancy. We apply such methods to the culture of both isolated, dispersed primary adipocytes and epididymal adipose explants. To facilitate more widespread adoption of the methodology, the devices presented here are designed for user-friendly operation. Only handheld syringes are needed to control flow, and devices are inexpensive and disposable. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 29% |
| Researcher | 4 | 13% |
| Student > Doctoral Student | 3 | 10% |
| Student > Master | 3 | 10% |
| Student > Postgraduate | 2 | 6% |
| Other | 5 | 16% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 26% |
| Chemistry | 5 | 16% |
| Agricultural and Biological Sciences | 4 | 13% |
| Engineering | 3 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 4 | 13% |
| Unknown | 6 | 19% |