Chapter title |
A Gold@Polydopamine Core–Shell Nanoprobe for Long-Term Intracellular Detection of MicroRNAs in Differentiating Stem Cells
|
---|---|
Chapter number | 10 |
Book title |
Biomedical Nanotechnology
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6840-4_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6838-1, 978-1-4939-6840-4
|
Authors |
Chun Kit K. Choi, Chung Hang J. Choi, Liming Bian |
Editors |
Sarah Hurst Petrosko, Emily S. Day |
Abstract |
MicroRNAs (miRNAs) represent an emerging class of biomarkers for studying and understanding biological events; the development of viable tools for detecting or monitoring the intracellular expression levels of specific miRNAs is of great interest to life scientists and biomedical engineers. Here, we describe the fabrication of a novel class of core-shell nanoprobes that comprise a gold nanoparticle core and a polydopamine (PDA) shell. Our nanoprobes can be used to specifically track the expression profiles of two miRNA markers of osteogenic differentiation (i.e., osteogenesis), namely, miR-29b and miR-31, in differentiating human mesenchymal stem cells (hMSCs). The newly designed nanoprobes may hold great promise in the noninvasive investigation of the long-term dynamics of cellular events such as stem cell differentiation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 29% |
Student > Doctoral Student | 2 | 10% |
Researcher | 2 | 10% |
Lecturer | 1 | 5% |
Student > Bachelor | 1 | 5% |
Other | 3 | 14% |
Unknown | 6 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 8 | 38% |
Biochemistry, Genetics and Molecular Biology | 2 | 10% |
Neuroscience | 1 | 5% |
Linguistics | 1 | 5% |
Materials Science | 1 | 5% |
Other | 1 | 5% |
Unknown | 7 | 33% |