Chapter title |
Ligand Exchange and 1H NMR Quantification of Single- and Mixed-Moiety Thiolated Ligand Shells on Gold Nanoparticles
|
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Chapter number | 2 |
Book title |
Biomedical Nanotechnology
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Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6840-4_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6838-1, 978-1-4939-6840-4
|
Authors |
Ashley M. Smith, Jill E. Millstone |
Editors |
Sarah Hurst Petrosko, Emily S. Day |
Abstract |
The use of nanoparticles in biomedicine critically depends on their surface chemistry. For metal nanoparticles, a common way to tune this surface chemistry is through mass action ligand exchange, where ligand exchange can be used to expand the functionality of the resulting nanoparticle conjugates. Specifically, the quantity, identity, and arrangement of the molecules in the resulting ligand shell each can be tuned significantly. Here, we describe methods to exchange and quantify thiolated and non-thiolated ligands on gold nanoparticle surfaces. Importantly, these strategies allow the quantification of multiple ligand types within a single ligand shell, simultaneously providing ligand composition and ligand density information. These results are crucial for both designing and assigning structure-function relationships in bio-functionalized nanoparticles, and these methods can be applied to a broad range of nanoparticle cores and ligand types including peptides, small molecule drugs, and oligonucleotides. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 75% |
Other | 1 | 25% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Chemistry | 1 | 25% |