Chapter title |
Identifying Novel Cancer Therapies Using Chemical Genetics and Zebrafish
|
---|---|
Chapter number | 5 |
Book title |
Cancer and Zebrafish
|
Published in |
Advances in experimental medicine and biology, May 2016
|
DOI | 10.1007/978-3-319-30654-4_5 |
Pubmed ID | |
Book ISBNs |
978-3-31-930652-0, 978-3-31-930654-4
|
Authors |
Michelle Dang, Rachel Fogley, Leonard I. Zon |
Editors |
David M. Langenau |
Abstract |
Chemical genetics is the use of small molecules to perturb biological pathways. This technique is a powerful tool for implicating genes and pathways in developmental programs and disease, and simultaneously provides a platform for the discovery of novel therapeutics. The zebrafish is an advantageous model for in vivo high-throughput small molecule screening due to translational appeal, high fecundity, and a unique set of developmental characteristics that support genetic manipulation, chemical treatment, and phenotype detection. Chemical genetic screens in zebrafish can identify hit compounds that target oncogenic processes-including cancer initiation and maintenance, metastasis, and angiogenesis-and may serve as cancer therapies. Notably, by combining drug discovery and animal testing, in vivo screening of small molecules in zebrafish has enabled rapid translation of hit anti-cancer compounds to the clinic, especially through the repurposing of FDA-approved drugs. Future technological advancements in automation and high-powered imaging, as well as the development and characterization of new mutant and transgenic lines, will expand the scope of chemical genetics in zebrafish. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 54 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 19% |
Student > Ph. D. Student | 10 | 19% |
Student > Master | 6 | 11% |
Student > Bachelor | 4 | 7% |
Student > Doctoral Student | 3 | 6% |
Other | 6 | 11% |
Unknown | 15 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 17 | 31% |
Agricultural and Biological Sciences | 7 | 13% |
Medicine and Dentistry | 6 | 11% |
Chemistry | 2 | 4% |
Neuroscience | 2 | 4% |
Other | 2 | 4% |
Unknown | 18 | 33% |