| Chapter title |
Protecting-Group-Free Synthesis of Glycopolymers and Their Binding Assay with Lectin and Influenza Virus
|
|---|---|
| Chapter number | 4 |
| Book title |
Macro-Glycoligands
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3130-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3129-3, 978-1-4939-3130-9
|
| Authors |
Tomonari Tanaka, Tadanobu Takahashi, Takashi Suzuki |
| Abstract |
Typical synthetic methods for glycopolymers are laborious and require multistep processes, including protection and deprotection steps. Here we describe a facile protecting-group-free synthetic approach to glycopolymers bearing oligosaccharides from free saccharides by direct azidation and click chemistry methods, followed by reversible addition-fragmentation chain transfer polymerization. This method can be applied not only to mono- and disaccharides, but also to large biologically relevant oligosaccharides having sialic acids. Due to the glycocluster effect, the glycopolymers strongly bind with the corresponding lectin and influenza A virus, as analyzed by the quartz crystal microbalance method and hemagglutination inhibition assay. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 20% |
| Researcher | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 2 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 60% |
| Chemical Engineering | 1 | 20% |
| Unknown | 1 | 20% |