| Chapter title |
Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting.
|
|---|---|
| Chapter number | 19 |
| Book title |
Oogenesis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3795-0_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3793-6, 978-1-4939-3795-0
|
| Authors |
Deanna M. Navaroli, Jonathan L. Tilly, Dori C. Woods |
| Editors |
Ioannis P. Nezis |
| Abstract |
The ability to isolate and subsequently culture mitotically active female germ cells from adult ovaries, referred to as either oogonial stem cells (OSCs) or adult female germline stem cells (aFGSCs), has provided a robust system to study female germ cell development under multiple experimental conditions, and in many species. Flow cytometry or fluorescence-activated cell sorting (FACS) is an integral part of many isolation and characterization protocols. Here, we provide methodological details for antibody-based flow cytometric isolation of OSCs using antibodies specific for external epitopes of the proteins Ddx4 or Ifitm3, alone or in combination with the use of fluorescent reporter mice. Beginning with sample preparation, we provide point-by-point instructions to guide researchers on how to isolate OSCs using flow cytometry. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 25% |
| Student > Bachelor | 3 | 19% |
| Student > Master | 2 | 13% |
| Researcher | 1 | 6% |
| Professor > Associate Professor | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Agricultural and Biological Sciences | 4 | 25% |
| Nursing and Health Professions | 1 | 6% |
| Materials Science | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 5 | 31% |