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Mendeley readers
| Chapter title |
Monitoring Calcium Oscillations in Fertilized Mouse Eggs.
|
|---|---|
| Chapter number | 17 |
| Book title |
Oogenesis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3795-0_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3793-6, 978-1-4939-3795-0
|
| Authors |
Guillaume Halet |
| Editors |
Ioannis P. Nezis |
| Abstract |
In mammalian species, including human, fertilization is characterized by the triggering of long-lasting calcium (Ca(2+)) oscillations in the egg cytoplasm. The monitoring of these Ca(2+) oscillations is a valuable technique to demonstrate that fertilization has occurred, to study egg activation events elicited downstream of the Ca(2+) signal, as well as to evaluate sperm quality. This chapter describes our protocol to monitor sperm-induced Ca(2+) oscillations in mouse eggs, using fluorescence microscopy techniques and the Fura-2-AM ratiometric Ca(2+) indicator. |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 2% |
| Student > Bachelor | 1 | 2% |
| Unknown | 39 | 95% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 5% |
| Unknown | 39 | 95% |